Molecular Study on plasmid profiling of clinical isolates of antibiotic resistant Klebsiella from Chittagong city

Jesmin Akter, AM Masudul Azad Chowdhury, Mohammad Al Forkan

Abstract


Klebsiella is a common cause of various infections. Plasmids are extra-chromosomal circular deoxyribonucleic acid molecules (DNA) that can exist and replicate independently of the chromosomes in bacteria.  In recent year’s Plasmid-mediated multi drug resistance (MDR) in different bacterial species are well recognized.  Plasmids can encode genes for antibiotic resistance or virulence factors and also serve as markers of various bacterial strains.  This study was done to observe the presence of plasmid in various multidrug resistant clinical isolates of Klebsiella from Chittagong city of Bangladesh.  To detect the molecular causes of multidrug resistance in Klebsiella, ten MDR Klebsiella isolates which showed mucoid phenotype on MacConkey media were selected for plasmid-DNA extraction procedure and agarose gel electrophoresis.  The present data showed that all the isolates were resistant to multiple antibiotics and among them, 70% Klebsiella isolates harboured plasmid.  The high occurrence of plasmid in MDR Klebsiella isolates indicates that plasmids might have epidemiological significance.

Keywords


Klebsiella; Nosocomial infection; Multidrug resistance (MDR)

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